Scutellaria barbate extract induces apoptosis of hepatoma H22 cells via the mitochondrial pathway involving caspase-3

Table 1 Effect of ESB on cell cycle and apoptosis of H22 cells by flow cytometry (mean ± SD)

Groups	n	G0/G1	S	G2/ M	Apoptosis rate
Control	5	37.63 ± 2.12	32.73 ± 2.24	21.75 ± 1.52	0.51 ± 0.12
ESB low dose	5	39.35 ± 2.25	33.56 ± 3.12	21.20 ± 1.27	1.07 ± 0.15
ESB medium dose	5	45.91 ± 2.56a	30.65 ± 2.64	17.15 ± 1.34a	3.15 ± 0.27a
ESB high dose	5	56.05 ± 2.37b	21.33 ± 3.42b	12.30 ± 1.25b	7.83 ± 0.43b

Cell cycle distributions in control and ESB-treated cells were determined by PI staining and flow cytometric analysis. Results presented were representative of three independent experiments.

^aP < 0.05,

^bP < 0.01 vs control group.