Effects of fluoxetine on mast cell morphology and protease-1 expression in gastric antrum in a rat model of depression

Figure 1 Gastric antrum tissue staining procedure include: (1) pretreatment with 0. 25% Triton X-100; (2) incubation in the primary antibody of rMCP-1; (3) incubation with secondary antibody (Cy3 -conjugated goat anti-rabbit IgG). A: Expression of rMCP-1 in the normal control group; B: Expression of rMCP-1 in the fluoxetine + normal control group; C: Expression of rMCP-1 in the depressed model control group; D: Expression of rMCP-1 in the saline + depressed model group; E: Expression of rMCP-1 in the fluoxetine + depressed model group.