An effective vaccine against colon cancer in mice: Use of recombinant adenovirus interleukin-12 transduced dendritic cells

Figure 6 Generation of tumor-specific cytotoxic T cells ex vivo. Splenic CD3⁺ T cells were magnetically isolated from naïve BALB/c mice. These cells were cultured ex vivo in the presence of cytokines including IL-2 and IL-7 and with CT26 TP AdVIL-12/DCs or CT26 TP DCs at a stimulator to responder cell ratio of 1:20. The primed T cells were effector cells, CT26 or SGC-7901 tumor cells were target cells. Then these effector T cells were titrated by serial dilution (E-T mix, E:T 1:1, 5:1, 10:1, 25:1, 50:1, 100:1), and their lytic activity against CT26 or SGC-7901 were assayed by a Cytotoxicity Detection Kit. Statistical analysis used the paired Student’s t test. Data are given as mean ± SE. A ^aP < 0.05, CT26 TP AdVIL-12/DCs vs CT26 TP DCs or other control groups in the CT26 groups.