Oxidative stress disturbs energy metabolism of mitochondria in ethanol-induced gastric mucosa injury

Table 1 Reacting system of RT

Component	Amount
25 mmol/L MgCl2	4.00 μL
Reverse transcription 10 × buffer	2.00 μL
10 mmol/L dNTP	2.00 μL
Recombinant ribonuclease inhibitor	0.50 μL
AMV reverse transcriptase	15.00 U
Oligo (dT) 15 primer	0.50 μg
Total RNA	1.00 μg
Nuclease-rree water	Proper volume
Reacting volume	20.00 μL