Oxidative stress disturbs energy metabolism of mitochondria in ethanol-induced gastric mucosa injury

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Oct 14, 2008 (publication date) through Apr 29, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Oct 14, 2008; 14(38): 5857-5867
Published online Oct 14, 2008. doi: 10.3748/wjg.14.5857

Table 1 Reacting system of RT

Component	Amount
25 mmol/L MgCl₂	4.00 μL
Reverse transcription 10 × buffer	2.00 μL
10 mmol/L dNTP	2.00 μL
Recombinant ribonuclease inhibitor	0.50 μL
AMV reverse transcriptase	15.00 U
Oligo (dT) 15 primer	0.50 μg
Total RNA	1.00 μg
Nuclease-rree water	Proper volume
Reacting volume	20.00 μL

Citation: Pan JS, He SZ, Xu HZ, Zhan XJ, Yang XN, Xiao HM, Shi HX, Ren JL. Oxidative stress disturbs energy metabolism of mitochondria in ethanol-induced gastric mucosa injury. World J Gastroenterol 2008; 14(38): 5857-5867
URL: https://www.wjgnet.com/1007-9327/full/v14/i38/5857.htm
DOI: https://dx.doi.org/10.3748/wjg.14.5857