Potentially probiotic bacteria induce efficient maturation but differential cytokine production in human monocyte-derived dendritic cells

Figure 4 Kinetics of probiotic bacteria induced cytokine gene expression in human moDCs. MoDCs were stimulated with GAS and indicated probiotic bacteria with a bacteria:host cell ratio of 40:1. A: Cells were collected at 8, 24, and 48 h after stimulation and total cellular RNA was isolated. mRNA expression was analyzed with Northern blotting using CCL20, TNF-α, IL-12p35, IFN-γ, and IL-10 cDNA probes. Ethidium bromide staining was used to control equal sample loading and the integrity of RNA; B: Cell culture supernatants from the experiment described in panel A were collected and cytokine levels in the supernatants were determined by ELISA. The bars and error bars represent the means and standard deviations from four different blood donors. One representative experiment out of two is shown. See Figure 3 for abbreviations of bacteria.