PCR-SSCP-DNA sequencing method in detecting PTEN gene mutation and its significance in human gastric cancer

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Jun 28, 2008 (publication date) through Apr 19, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Gastric Cancer

World J Gastroenterol. Jun 28, 2008; 14(24): 3804-3811
Published online Jun 28, 2008. doi: 10.3748/wjg.14.3804

Table 1 Primer sequence, length and annealing temperature of exons 5-8 in PTEN gene

	Primer sequence	Primer length (bp)	Amplification fragment length (bp)	Annealing temperature (°C)
Exon-5F:	ACCTGTTAAGTTTGTATGCAAC	22	379	52
R	TCCAGGAAGAGGAAAGGAAA	20
Exon-6F:	CATAGCAATTTAGTGAAATAACT	23	274	52
R	GATATGGTTAAGAAAACTGTTC	22
Exon-7F:	TGACAGTTTGACAGTTAAAGG	21	263	58
R	GGATATTTCTCCCAATGAAAG	21
Exon-8F:	CTCAGATTGCCTTATAATAGTC	22	558	52
R	TCTGTTACTTGCTACGTAAAC	21

Citation: Guo CY, Xu XF, Wu JY, Liu SF. PCR-SSCP-DNA sequencing method in detecting PTEN gene mutation and its significance in human gastric cancer. World J Gastroenterol 2008; 14(24): 3804-3811
URL: https://www.wjgnet.com/1007-9327/full/v14/i24/3804.htm
DOI: https://dx.doi.org/10.3748/wjg.14.3804