Negundoside, an iridiod glycoside from leaves of Vitex negundo, protects human liver cells against calcium-mediated toxicity induced by carbon tetrachloride

Figure 16 Effect of NG and silymarin on CCl₄-induced decrease in GSH levels. HuH-7 cells were pre-incubated with medium containing test materials (NG and silymarin) for 1 h. The cultures were then further incubated in presence and absence of CCl₄ for 24 h. The cells were harvested by scraping and GSH levels were determined as described under Materials and Methods section. BSO (100 &mgr;mol/L) was used as positive control. Data are expressed as mean ± SD and are from representative experiments repeated twice and conducted in triplicate. Statistical significance: ^bP < 0.001 vs the untreated control; ^dP < 0.01; ^fP < 0.001; NS = non-significant vs CCl₄-treated cells.