Negundoside, an iridiod glycoside from leaves of Vitex negundo, protects human liver cells against calcium-mediated toxicity induced by carbon tetrachloride

Figure 13 Effect of NG and silymarin against CCl₄-induced cell cycle arrest. HuH-7 cells were pre-incubated for 1 h with test materials (NG and silymarin) at mentioned concentrations. The cells were further incubated for 24 h with CCl₄ (2 mmol/L). Thereafter, cells were harvested by trypsinization, fixed with ethanol, stained with PI , and analyzed using flow cytometry. Representative histograms are shown, and the percentage of cells in the sub G₀/G₁ fraction (M1 zone, hypodiploid area) are shown.