Negundoside, an iridiod glycoside from leaves of Vitex negundo, protects human liver cells against calcium-mediated toxicity induced by carbon tetrachloride

Figure 11 Effect of NG and silymarin against CCl₄-induced loss of mitochondrial membrane potential (ΔΨm). HuH-7 cells were pre-incubated for 1 h with test materials (NG and silymarin) at mentioned concentrations. The cells were further incubated for 24 h with CCl₄ (2 mmol/L). Thereafter, cells were stained with Rhodamine-123 and analysed by flow cytometry as described in Materials and Methods section. Representative histograms are shown and the percentage of cells in depolarized zone (M1 zone) are shown.