Secretory Transactivating Transcription-apoptin fusion protein induces apoptosis in hepatocellular carcinoma HepG2 cells

Figure 4 Cell death induced by secreted TAT-apoptin. A: HepG2 cells immunostained 4 h after co-culture with the supernatant of CHO cells expressing SP-TAT-apoptin. Recombinant TAT-apoptin detected by anti-V5-FITC antibody is shown in green and cell nuclei stained by PI in red (× 1000); B: Translocation of recombinant apoptin in HepG2 cells 24 h after co-culture with the supernatant of CHO cell expressing SP-TAT-apoptin; C: Cell viability determined by MTT dye reduction assay after co-culture with secreted TAT-apoptin.