Secretory Transactivating Transcription-apoptin fusion protein induces apoptosis in hepatocellular carcinoma HepG2 cells

Figure 2 SP-TAT-apoptin-induced cell death. A: Cell viability measured by flow cytometry. A1 and A2: HUVECs at 48 and 72 h post-transfection; A3-A6: HepG2 cells at 24, 48, 72 and 96 h. HepG2 cells were susceptible to SP-TAT-apoptin-induced apoptosis in a time-dependent manner; B: Cell viability determined by MTT dye reduction assay; C: DNA fragmentation in HepG2 cells demonstrated by agarose gel electrophoresis. Lane 1: 1 kb DNA marker; Lanes 2-5: DNA from cells at 24, 48, 60 and 72 h post-transfection, respectively.