Effect of Chaiqinchengqi decoction on sarco/endoplasmic reticulum Ca2+-ATPase mRNA expression of pancreatic tissues in acute pancreatitis rats

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Apr 21, 2008 (publication date) through Apr 23, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Apr 21, 2008; 14(15): 2343-2348
Published online Apr 21, 2008. doi: 10.3748/wjg.14.2343

Table 1 Probe, primer and product (bp) in RT-PCR

Genes	Probes	Upstream primers	Downstream primers	Product (bp)
SERCA1	5’-CGATGTCCCGAGCCTTGATCC-3’	5’-GGTTTGGCAGGAACGGAAT-3’	5’-GGTGGATTTGATGGAGAGGAT-3’	199
SERCA2	5’-CACACTCTTTCTGTCCTGTCG-3’	5’-ATGAACCTGAAATGGGCAAG-3’	5’-GGAACTTTGTCACCAACAGCA-3’	115
GAPDH	5’-FAM-ACCACAGTCCATGCCATCAC-TAMRA-3’	5’-CCTCAAGATTGTCAGCAAT-3’	5’-CCATCCACAGTCTTCTGAGT-3’	141

SERCA: Sarco/endoplasmic reticulum Ca²⁺-ATPase; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.

Citation: Xue P, Deng LH, Zhang ZD, Yang XN, Xia Q, Xiang DK, Huang L, Wan MH. Effect of Chaiqinchengqi decoction on sarco/endoplasmic reticulum Ca²⁺-ATPase mRNA expression of pancreatic tissues in acute pancreatitis rats. World J Gastroenterol 2008; 14(15): 2343-2348
URL: https://www.wjgnet.com/1007-9327/full/v14/i15/2343.htm
DOI: https://dx.doi.org/10.3748/wjg.14.2343