A red wine polyphenolic extract reduces the activation phenotype of cultured human liver myofibroblasts

Figure 4 Effect of the RWPE on MMP-2 and TIMP-1 expression. A: Myofibroblasts were incubated for 24 h with the indicated concentrations of RWPE (in &mgr;g/mL). Aliquots of conditioned medium normalized for the DNA content of the cell monolayers were analyzed on gelatin-containing gels (A). The white bands on the dark background indicate gelatinolytic activity. Other aliquots were analyzed by Western blot with an antibody against TIMP-1 (B). Another experiment gave similar results; C: Quantitative analysis of the experiments. MMP-2: Mean of duplicate samples; TIMP-1: Mean of 2 independent experiments.