Immune-mediated anti-neoplastic effect of intratumoral RSV envelope glycoprotein expression is related to apoptotic death of tumor cells but not to the size of syncytia

Figure 5 Analysis of apoptosis. A: MC38 or Colon26 cells were transduced in vitro with indicated adenovirus vectors and early apoptotic events were analyzed by flow cytometric measurements of phosphatidylserine translocation to the outer membrane by annexin V binding; B: Because apoptosis is essentially executed by proteases of the caspase family, we analyzed caspase-3/7 activity. Relative caspase activities are given as means ± SD of three independent experiments; C: To measure mitochondrial alterations we determined the mitochondrial membrane potential ΔΨ_m. Ad.Null served as a control.