Construction of recombinant attenuated Salmonella typhimurium DNA vaccine expressing H pylori ureB and IL-2

Figure 3 Agarose gel electrophoresis analysis of recombinant attenuated Salmonella typhimurium DNA vaccine strain with restriction enzyme digestion (A) (lane1: pIRES after digestion with Nhe I and Xho I, lanes 2-3: recombinant plasmid pIRES-ureB-IL-2 from strains of different generations after digestion with Nhe I and Xho I, lanes 4-5: pIRES-ureB-IL-2 from strains of different generations after digestion with Sal I and Not I, lane 6: DNA marker: (DL2000 + 15 000) and identification of recombinant attenuated Salmonella typhimurium DNA vaccine strain carrying ureB by PCR (B) (lane1: product amplified from pIRES as a negative control, Lane4: Marker (DL2000); Lanes 2-3: ureB amplified from strains of different generations, lane 4: marker-DL2000, lanes 5-6: IL-2 amplified from strains of different generations.