Calcitriol analog ZK191784 ameliorates acute and chronic dextran sodium sulfate-induced colitis by modulation of intestinal dendritic cell numbers and phenotype

Figure 4 Effects of ZK191784 treatment on the secretion of cytokines and expression of costimulatory molecules by BM-DCs. DCs were generated from bone marrow of mice and stimulated with LPS (1 μg/mL) overnight in the presence of different concentrations of ZK191784 (10, 100 or 1000 nmol/L). Concentrations of the proinflammatory cytokines IFN-γ (A) and IL-12 (B) were measured within the supernatants by ELISA. ^aP < 0.05, ^cP < 0.05, data presented are representative of three independent experiments. BM-DCs were cultured in the presence of ZK191784 (1000 nmol/L) or vehicle, and were stimulated overnight with LPS. CD11c⁺ DCs were stained with FITC-conjugated mAbs for expression of the costimulatory molecules CD40, CD80 and CD86. Isotype controls are shown with dark lines, and positive staining is shown in grey (C).