Effects and mechanisms of silibinin on human hepatoma cell lines

Figure 1 Silibinin's effects on growth of human hepatoma cells. A: MTT assay showed silibinin's dose dependent anti-proliferative effects on HuH7 cells. A significant decrease in proliferation compared to control was noted from silibinin ≥ 180 μmol/L. The IC₂₅ is determined to be 120 μmol/L and the IC₅₀ is determined to be 240 μmol/L; B: Silibinin's effects on other human HCC cell growth. PLC/PRF/5, HepG2, and Hep3B HCC cells were treated with silibinin at IC₂₅ and IC₅₀ doses for HuH7 cells. Silibinin significantly reduced growth of all three HCC cells in different rates. ^aP < 0.05 vs control.