Serum-free culture of H pylori intensifies cytotoxicity

Figure 3 Cell viability assay of the supernatant and the extract of serum-free cultured H pylori at the onset of culturing and long cultured H pylori, approximately 100 passages after the onset of culturing. A: Long serum-free cultured supernatant showing dose-dependent decrease curves of cell viability; B: Long serum-free cultured extract showing further dose-dependent decrease curves of cell viability. Each point represents the mean ± SD from three separate experiments.