Involvement of Krüppel-like factor 6 (KLF6) mutation in the development of nonpolypoid colorectal carcinoma

Table 3 Individual data for genetic analysis of 55 nonpolypoid colorectal tumors

	KLF6		p53		K-ras	B-raf
Case	LOH1	Mutation	IHC2	LOH4	Mutation	Mutation
Intramucosal cancer
16	NI3	Wild	(-)	NI	GGC to GCC [13, Gly to Ala (1/4)]	Wild
17	(-)		(-)		Wild	Wild
29	LOH (3/3)	Wild	(-)		Wild	Wild
30	LOH (2/4)	Wild	(-)	(-)	Wild	Wild
31		Wild	(-)	(-)	Wild	Wild
32		Wild	(++)	LOH (3/3)	Wild	Wild
33	(-)		(+)	LOH (1/3)	Wild	AAA to CAA [560, Lys to Gln (1/3)]
34	(-)		(+++)		Wild	Wild
35		Wild	(-)	LOH (1/3)	Wild	Wild
36	(-)		(-)	LOH (1/4)	Wild	Wild
Submucosal cancer
1	LOH (1/4)	Wild	(-)		Wild	Wild
2		Wild	(-)	(-)	Wild	Wild
3	NI	Wild	(+)		GGC to GCC [13, Gly to Ala (1/4)]	CAT to CTT [396, His to Leu (1/4)]
4	(-)		(+++)	LOH (1/5)	Wild	Wild
5		Wild	(+++)	(-)	Wild	Wild
6	NI	Wild	(-)	LOH (2/3)	Wild	Wild
7	NI		(+++)	(-)	Wild	Wild
8	LOH (2/3)	Wild	(++)	(-)	Wild	Wild
9	NI	Wild	(+++)		Wild	Wild
10	NI	Wild	(-)	LOH (2/3)	Wild	Wild
11	NI		(+++)	(-)	Wild	Wild
12	NI		(+++)	LOH (2/4)	Wild	Wild
13	LOH (3/7)	Wild	(+++)	(-)	Wild	Wild
14	NI	Wild	(-)	(-)	Wild	Wild
15	LOH (3/4)	Wild	(+++)		Wild	Wild
18	(-)		(+)		Wild	Wild
19	NI		(-)		Wild	Wild
20		Wild	(-)		GGC to GCC [13, Gly to Ala (1/4)]	CAG to CCG [608, Glu to Pro (1/4)]
21	NI	Wild	(++)	(-)	Wild	GTG to ATG [378, Val to Met (1/3)]
22	LOH (2/4)	CTG to GCT [735, Leu to Ala (2/4)]	(++)		Wild	Wild
23	NI	CTG to GCT [735, Leu to Ala (2/4)]	(+)	(-)	Wild	Wild
24	NI	Wild	(-)	(-)	GGC to GCC [13, Gly to Ala (1/5)]	Wild
25			(+)		Wild	AAA to CAA [560, Lys to Gln (1/5)]
26	(-)	Wild	(-)		Wild	AAA to CAA [560, Lys to Gln (2/6)]
27	(-)		(-)	NI	Wild	Wild
28		Wild	(-)		Wild	Wild
37	(-)		(-)	LOH (4/4)	Wild	Wild
38	(-)		(+++)	LOH (4/4)	Wild	Wild
39	(-)		(-)		Wild	Wild
40	(-)		(++)	(-)	Wild	Wild
41		Wild	(-)		Wild	Wild
42			(-)		Wild	Wild
43	(-)		(++)		Wild	Wild
44	LOH (3/3)	Wild	(-)	NI	Wild	Wild
45	LOH (1/5)	Wild	(+++)	LOH (5/5)	Wild	Wild
46	(-)		(+++)	(-)	Wild	Wild
47	LOH (3/3)	Wild	(-)	(-)	Wild	Wild
48	LOH (2/4)	Wild	(-)		Wild	Wild
49		Wild	(-)	(-)	GGC to GCC [13, Gly to Ala (1/4)]	Wild
50		Wild	(-)	LOH (3/5)	Wild	Wild
51	(-)		(-)	(-)	Wild	Wild
52	LOH (3/4)	Wild	(-)		Wild	Wild
53			(-)	LOH (1/4)	Wild	Wild
54	LOH (1/5)	Wild	(-)		Wild	Wild
55	LOH (1/4)	Wild	(+++)	LOH (3/4)	Wild	Wild

¹LOH: loss of heterozygosity;

²IHC: immunohistochemistry;

³NI: not informative; blank space, not examined;

⁴Number of LOH or mutation-positive foci per number of microdissected tumor foci is indicated in parenthesis.