Involvement of Krüppel-like factor 6 (KLF6) mutation in the development of nonpolypoid colorectal carcinoma

Table 1 PCR primer sets

Priming region		Primer sequence
Loss of heterozygosity analysis
	p53 (TP53)	5′-AGGGATACTATTCAGCCCGAGGTG-3′
		5′-ACTGCCACTCCTTGCCCCATTC-3′
	KLF6 (D10S591)	5′-ACCTCGAAGGTCTGTTCTCC-3′
		5′-GGCTTTATGGATCATATTAATCCAC-3′
	KLF6 (D10S594)	5′-GGGCAGCGTTGCTGAGA-3′
		5′-GCACCCAGATAGGCATAGAGA-3′
Mutation analysis
	K-ras exon 1:	5′-GGCCTGCTGAAAATGACTGA-3′
		5′-GGTGCAGGACCATTCTTTGAT-3′
	B-raf exon 11:	5′-AAACACTTGGTAGACGGGAC-3′
		5′-AATGTGGTGACATTGTGACAAGT-3′
	B-raf exon 15:	5′-CTTCATGAAGACCTCACAGT-3′
		5′-TCCACTGATTAAATTTTTGGCC-3′
KLF6	exon 1:	5′-GCCTCGCCGCCCTCGC-3′
		5′-AACTCCAAACAGCCGACCC-3′
	exon 2:	5′-CAATCACGTGCCTTCTCTGG-3′
		5′-GAGAAAGTGAGGATTTGTCTG-3′
	exon 3	5′-GTCTGCGGGTCAGTGAAGTC-3′
		5′-GTCATCACATTCCCAAGGCC-3′