Roles of the MEK1/2 and AKT pathways in CXCL12/CXCR4 induced cholangiocarcinoma cell invasion

Figure 7 A: Effect of U0126 and LY294002 on CXCL12-induced phosphorylation of MEK1/2 and Akt in cholangiocarcinoma cells. RMCCA1 cells were pre-treated with U0126 or LY294002 before added CXCL12. Cells were collected at 5 min and MEK1/2 and Akt phosphorylation were determined by Western blot as described; B: The average band intensity based on 3 biologically separate experiments, showing levels of MEK1/2 and Akt phosphorylation relative to the levels of MEK1/2 and Akt expression from the same experiment (^aP < 0.05 compared with control); C: Effect of MEK1/2 and Akt phosphorylation induced by CXCL12 on the invasion of cholangiocarcinoma cells. RMCCA1 and KKU100 were pre-treated with or without LY294002 and U0126 for 12 h then were seeded in the 24-well Biocoat Matrigel invasion chamber. The bottom chamber contained 0 or 100 ng/mL of CXCL12. After 24 h, the cells on the lower surface were assayed as described previously. LY294002 and U0126 inhibited the effect of CXCL12 induced cholangiocarcinoma cells invasion (^aP < 0.05).