Effects of Terminalia arjuna bark extract on apoptosis of human hepatoma cell line HepG2

Figure 4 Expression of p53 and caspase-3 proteins. Western blotting analysis of p53 and caspase-3 in HepG2 cells cultured for 48 h in the presence and absence of T. arjuna extract at the concentration 60 and 100 mg/L was carried out. Cell lysates were prepared as described in MATERIALS AND METHODS, and resolved by 12% SDS-PAGE, immunoblotted and detected using specific antibodies which recognized p53 (A) and caspase-3 (B). Densitometric analysis of the p53 (C) and caspase-3 band (D) was performed. Each value is mean ± SD of three observations. ^aP < 0.05 represents the statistical significance between control and T. arjuna extract-treated HepG2 cells.