Loss of heterozygosity of Kras2 gene on 12p12-13 in Chinese colon carcinoma patients

Table 1 Primer sequences on 12p12-13 microsatellite markers

Markers	Primer sequences	Length(bp)
D12S89	D12S89-F:5’-ATTTGAGAGCAGCGTGTTTT-3’	254-288
	D12S89-R:5’-CCATTATGGGGAGTAGGGGT-3’
D12s358	D12s358-F:5’-GCCTTTGGGAAACTTTGG-3’	238-270
	D12s358-R:5’-TAAGCCCTTTTATTTTTCCTAAC-3’
D12s310	D12s310-F:5’-GAAAACTAATTGCCCCTTAC-3’	243-253
	D12s310-R:5’-TTTAGATTCTCCAAATGCC-3’
D12S1606	D12S1606-F:5’-ATGAGAGGCCAAATTAAAG-3’	271-279
	D12S1606-R:5’-CTACTGTTGTGTCAGGGTCA-3’
D12s1596	D12s1596-F:5’-TCATGTGGCTGGTAGAGAAG-3’	204-222
	D12s1596-R:5’-CGTGAAGCAGATTTATCGTG-3’
D12s1617	D12s1617-F:5’-AGCCTGAGGGGCCACAT-3’	215-261
	D12s1617-R:5’-TGGGCAACTTGGATAAGAAACA-3’
D12s1592	D12s1592-F:5’-GCTGAGTGTGGTGGCAC-3’	238-280
	D12s1592-R:5’-GAGACTATTCCAACAGTGTATTTTC-3’
D12S1057	D12S1057-F:5’-AGAGACAGAAGCAGTAGGATGG-3’	227
	D12S1057-R:5’-GGTTCTGACGTTTTAAGACTCG-3’
D12S1591	D12S1591-F:5’-GCGCTTTGTACTATGTTACATTT-3’	234-276
	D12S1591-R:5’-GAAGACGTTGGGTGAATC-3’
D12S823	D12S823-F:5’-GGGCAATAGAGTGAGATTCTG-3’	119
	D12S823-R:5’-CCTACCTCCTTCCCCATCC-3’
D12S1034	D12S1034-F:5’-TTTTCAAAAAGTGTGACTGTGC-3’	280-302
	D12S1034-R:5’-CAGCCTAGTAAAAAATTTAAATTGG-3’