GFAP promoter directs lacZ expression specifically in a rat hepatic stellate cell line

Figure 5 Induction of GFAP-lacZ transgene expression by TGF-β1. The T6/lacZ/C1 cells were cultured and incubated in full DMEM supplemented with 0 (A), 0.1 (B), 1 (C), and 10 (D) ng/mL of TGF-β1 for 3 days, and stained with X-gal substrate for 1 h. Cells treated TGF-β1 displayed more intense blue staining and more activated morphology (thicken processes). Scale bar = 60 µm.