N-terminal and C-terminal cytosine deaminase domain of APOBEC3G inhibit hepatitis B virus replication

Figure 1 Construction of APOBEC3G and its N-terminal and C-terminal cytosine deaminase domain expression plasmids. Full-length APOBEC3G sequence was cloned into EcoRI/HindIII restriction sites of the CMV-driven expression vector fused with a hemagglutinin fusion epitope tag at its N- terminal end (pXF3H) to construct APOBEC3G expression plasmid (pA3G). pA3G was digested with EcoRI/BamHIand BamHI/HindIII, these fragments were inserted into pXF3H to construct the N-terminal and C-terminal cytosine deaminase domain expression plasmids (pXFNA3G and pXFCA3G), respectively. pA3G was digested with EcoRI/BglII, and used for the construction of the N-terminal region of APOBEC3G which did not contain any cytosine deaminase domain plasmid (pXFEA3G). pCMV: CMV promoter; CD1: N-terminal cytosine deaminase domain; CD2: C-terminal cytosine deaminase domain.