A single dose of caffeic acid phenethyl ester prevents initiation in a medium-term rat hepatocarcinogenesis model

Figure 2 Effect of CAPE on GGT expression. A: GGT histochemically stained sections (1) non-treated group (NT), (2) carcinogenic treatment group (CT), (3) CT plus CAPE before DEN; B: CAPE effect on number/cm² of AHF and percentage of GGT⁺ area/tissue. The differences of AHF number and area after the effect of CAPE in these groups were compared with the respective control group (CT + vehicle) and obtained values were significant by t with ^aP < 0.05. Twelve histological liver sections per rat from each treatment were randomly selected. NT (n = 3), CT (n = 4), CT plus CAPE (n = 7); C: The RT-PCR assay was used to determine GGT mRNA expression. L1: Control NT; L1: CT group; L1: CT plus CAPE. The series of the extreme right represents α-actin transcripts as control. This experiment was performed with a pool of total RNA isolated from 3 animals.