Signaling pathway of insulin-like growth factor-II as a target of molecular therapy for hepatoblastoma

Figure 5 Huh-6 and HepG2 dead due to apoptosis. HE staining was performed to analyze morphological changes after addition of inhibitors. Many Huh-6 cells were dead treated with PPP (60 μmol/L), LY294002 (50 μmol/L), or Wortmannin (200 μmol/L) while HepG2 with PPP, LY294002, Wortmannin, or PD98059 (20 μmol/L). Most of the dead cells had pyknotic or fragmented nuclei (arrows), suggesting apoptosis. Areas indicated by arrows were magnified (x 400).