Hydrodynamics based transfection in normal and fibrotic rats

Figure 6 400 μg of pGL3-Control plasmid was injected in 5. 25% (volume per animal weight) Ringer in 5-8 s to normal rats, and rats previously injected intraperitoneally twice weekly with thioacetamide (TAA) for 3 wk. Prior to the hydrodynamic injection the rats were fed for 3 wk with either control food or food containing halofuginone at a concentration of 5 ppm. Twenty four hours post HBT the liver was excised and 200 mg samples of the median lobe were homogenized in reporter lysis buffer and the activity of luciferase monitored (n = 5).