Induction of apoptosis on human hepatocarcinoma cell lines by an alkyl resorcinol isolated from Lithraea molleoides

Figure 5 Treatment with 5-AR induces nuclear condensation and fragmentation in HepG2 and Hep3B cells. Cells were stained with acridine orange and propidium iodide, and observed under fluorescent microscopy. A: Control HepG2 cells (100 x magnification); B: treated HepG2 cells (100 x magnification); C: treated HepG2 cells (200 x magnification), showing in detail morphological changes of nuclear chromatin; D: control Hep3B cells (100 x magnification); E: treated Hep3B cells (100 x magnification); F: treated Hep3B cells (200 x magnification). Results are from one experiment that is representative of three similar ones.