Colorectal Cancer
Copyright ©2006 Baishideng Publishing Group Co.
World J Gastroenterol. Sep 21, 2006; 12(35): 5635-5643
Published online Sep 21, 2006. doi: 10.3748/wjg.v12.i35.5635
Figure 7
Figure 7 Effects of NVP-AEW541 on the expression and phosphorylation of apoptosis- and cell cycle- related proteins. Modulation of protein phosphorylation or protein expression by NVP-AEW541 treatment was analyzed by Western blotting. A: NVP-AEW541 treatment (24 h) induced a dose-dependent dephosphorylation of mitogenic ERK1/2; B: NVP-AEW541 dose-dependently increased the expression of the proapoptotic Bax protein, while the expression of the antiapoptotic protein Bcl-2 slightly decreased. The cell cycle promoter cyclin D1 was down-regulated by NVP-AEW541 treatment, while the cell cycle inhibitors p21Waf1/CIP1 and p27Kip1 were up-regulated. Moreover, COX-2 expression was down-regulated by NVP-AEW541. Expression of β-actin was used as a loading control.