Tyrosine kinase of insulin-like growth factor receptor as target for novel treatment and prevention strategies of colorectal cancer

Figure 2 Effects of NVP-AEW541 on HT-29 (A) and HCT-116 (B) cell growth as well as induction of cell death and morphological changes of isolated primary colorectal cancer cells (C). After 4 d of incubation with rising concentrations of NVP-AEW541, the number of HT-29 (A) and HCT-116 (B) cells decreased by > 95%, as determined by crystal violet staining (mean ± SE, n = 4). In both cell lines statistical significance (P < 0.05) of growth inhibition by NVP-AEW541 was shown for concentrations of 0.5-10 μmol/L. After 3 d of incubation with 0-5 μmol/L NVP-AEW541, the induction of cell death and morphological changes of isolated primary colorectal cancer cells was determined by Live/Dead-fluorescence microscopy (C). Viable cells are stained green, while cells with impaired cell membrane appear red. Phase-contrast images and corresponding fluorescence micrographs of a representative preparation (out of 6 NVP-AEW541-sensitive primary cell cultures) are depicted.