Gene therapy that inhibits NF-κB results in apoptosis of human hepatocarcinoma by recombinant adenovirus

Figure 1 M: DNA Marker; Lanes 1, 3: Recombinant adenoviral plasmids pAd-IκBαM/pAd-IκBα un-cleaved with PacI; Lanes 2, 4: Recombinant adenoviral plasmids pAd-IκBαM/pAd-IκBα cleaved with PacI. A: Indentification of recombinant adenoviral plasmids by restriction analysis; B, C: The pAd-IκBαM/pAd-IκBα was transfected into 293 cells and GFP expression was observed by fluorescence microscopy after transfection for 36 h; B: pAd-IκBαM; C: pAd-IκBα (× 200). D: The recombinant adenovirus AdIκBαM infected into HepG₂ and GFP expression was visualized by fluorescence microscopy after transfection for 48 h, (× 200).