1,2,3,4,6-penta-O-galloyl-β-D-glucose up-regulates heme oxygenase-1 expression by stimulating Nrf2 nuclear translocation in an extracellular signal-regulated kinase-dependent manner in HepG2 cells

Figure 5 Effects of PGG-induced ERK activation on HO-1 expression and t-Bt-HP-induced toxicity in HepG2 cells. A: Cells were incubated with 20 µmol/L PGG for indicated times, and Western blotting was performed with specific antibodies; B: Cells were incubated with 20 µmol/L PGG for 12 h in the presence or absence of 10 µmol/L U0123 (UO), 20 µmol/L SB203580 (SB), or 20 µmol/L SP600125 (SP), and Western blotting was performed with HO-1 antibody; C: Cells untreated or treated with PGG in the presence or absence of each specific inhibitor for 12 h were exposed to 100 µmol/L t-Bt-HP for 4 h. Data are expressed as mean ± SE of five independent experiments.