Reconstruction of liver organoid using a bioreactor

Figure 5 Scanning electron microscopic image of the surface of sinusoidal endothelial cells. A: Low-magnification scanning electron microscopic images of the surface of sinusoidal endothelial cells cultured on plastic dishes. The sinusoidal endothelial cells formed a thin layer on the plastic dish substrate. Scale bar: 5 μm; B: High-magnification scanning electron microscopy images of the surface of sinusoidal endothelial cells cultured on plastic dishes. Fenestrae could not be detected on the surface of endothelial cells. Only small pits are seen (arrow). Scale bar: 1 μm; C: Low-magnification scanning electron microscopic view of the surface of sinusoidal endothelial cells cocultured in the RFB. Fenestrated pores could be observed (arrow). Scale bar: 5 μm; D: High-magnification scanning electron microscopic view of the surface of sinusoidal endothelial cells cocultured in the RFB. Pores have a diameter of 100 - 200 nm. Scale bar: 1 μm.