Effects of Guiyuanfang and autologous transplantation of bone marrow stem cells on rats with liver fibrosis

Figure 4 Fate of transplanted BMSCs 4 wk after transplantation. The differentiation of transplanted BMSCs in liver fibrosis environment. A: Some transplanted stem cells have migrated into parenchyma, exhibiting the morphology of hepatocyte (BMSCs group, 200×); B: Most transplanted cells were distributed at portal region (Guiyuanfang plus BMSC group, 200×); C: Some transplanted stem cells exhibited the morphology of bile epithelium cells or hepatocyte (Guiyuanfang plus BMSC group, 400×); D: Double immunohistochemical staining showed that the BrdU-labeled cells were fully integrated in the liver parenchyma, along with the expression of cytokeratin-18, the liver epithelial specific marker (Guiyuanfang plus BMSCs group, 400×).