Bacterial biota in reflux esophagitis and Barrett’s esophagus

Figure 1 Microscopic examination of bacterial cells in the esophagus. Esophageal biopsies were fixed in formalin, paraffin-embedded, sectioned, and examined by using Gram-Twort stain. A: In the biopsy from patient #265 with a normal esophagus, Gram-negative cocci and coccobacilli were tightly associated with the surface of squamous epithelial cells. B: In the biopsy from patient #246 with Barrett’s esophagus, Gram-positive cocci were highly concentrated within the lumen of an intestinal-type gland.