Using the polymerase chain reaction coupled with denaturing gradient gel electrophoresis to investigate the association between bacterial translocation and systemic inflammatory response syndrome in predicted acute severe pancreatitis

Table 1 Oligonucleotide primers used for amplification of total DNA extracted from human blood samples and total chromosomal DNA recovered from selected bacterial strains

Primer designate	Sequences of (+) and (-) primers (nucleotide)	Gene target	Size of amplicon (bp)
BG-1 (+ strand)	5’CTT TGC CTG GTT TCC GGC ACC AGA A- 3’ (201-225)	b-Galactosidase gene of E coli	762
BG-4 (- strand)	5’AAC CAC CGC ACG ATA GAG ATT CGG G- 3’ (983-939)
BD-1 (+ strand)	5’AGT TTG ATC CTG GCT GAG- 3’ (8-27)	DNA coding for 16S rRNA	798
BD-2 (- strand)	5’GGA CTA CCA GGG TAT CTA AT- 3’ (805-787)
BFR-1 (+ strand)	5’ACT CTT TGT ATC CCG ACG ATT-3’ (484-504)	Glutamine synthase gene of Bacteroides spp.	581
BFR-2 (- strand)	5’GAG GTT GAT GCC TGT ATC GGT-3’ (1 065-1 045)
F3 (+ strand)	5’CGCCCGCCGCGCGCGGCGGGCGGGGCGGGGGCACGGGGGGCCTACGGGAGGCAGCAG-3’	Variable V3 region of 16S rRNA	233
Rev-2 (- strand)	5’ATTACCGCGGCTGCTGG-3’