Remes-Troche JM, Duarte-Rojo A, Morales G, Robles-Díaz G. Hemoconcentration is a poor predictor of severity in acute pancreatitis. World J Gastroenterol 2005; 11(44): 7018-7023
Corresponding Author of This Article
Professor Guillermo Robles-Díaz, Department of Experimental Medicine, Facultad de Medicina, Universidad Nacional Autónoma de México, Hospital General de México, Dr Balmis # 148, Colonia de los Doctores, CP 06726, México City, Mexico. email@example.com
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
World J Gastroenterol. Nov 28, 2005; 11(44): 7018-7023 Published online Nov 28, 2005. doi: 10.3748/WJG.v11.i44.7018
Hemoconcentration is a poor predictor of severity in acute pancreatitis
José M. Remes-Troche, Andrés Duarte-Rojo, Gustavo Morales, Guillermo Robles-Díaz
José M. Remes-Troche, Andrés Duarte-Rojo, Gustavo Morales, Department of Gastroenterology, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Vasco de Quiroga # 15, Colonia Seccion XVI, Tlalpan, CP 14000, Mexico City, Mexico
Guillermo Robles-Díaz, Department of Experimental Medicine, Facultad de Medicina, Universidad Nacional Autónoma de México, Hospital General de México, Dr Balmis # 148, Colonia de los Doctores, CP 06726, México City, Mexico
Author contributions: All authors contributed equally to the work.
Correspondence to: Professor Guillermo Robles-Díaz, Department of Experimental Medicine, Facultad de Medicina, Universidad Nacional Autónoma de México, Hospital General de México, Dr Balmis # 148, Colonia de los Doctores, CP 06726, México City, Mexico. firstname.lastname@example.org
Telephone: +52-55-56232673 Fax: +52-55-56232673
Received: April 7, 2005 Revised: April 23, 2005 Accepted: April 26, 2005 Published online: November 28, 2005
AIM: To determine whether the hematocrit (Hct) at admission or at 24 h after admission was associated with severe acute pancreatitis (AP), organ failure (OF), and pancreatic necrosis.
METHODS: A total of 336 consecutive patients with a first AP episode were studied. Etiology, Hct values at admission and at 24 h, development of severe AP according to Atlanta’s criteria, pancreatic necrosis, OF and mortality were recorded. Hemoconcentration was defined as Hct level >44% for males and >40% for females. The t-test and χ2 test were used to assess the association of hemoconcentration to the severity, necrosis and OF. Diagnostic accuracy was also determined.
RESULTS: Biliary disease was the most frequent etiology (n = 148). Mean Hct levels at admission were 41±6% for females and 46±7% for males (P<0.01). Seventy-eight (23%) patients had severe AP, and OF developed in 45 (13%) patients. According to contrast-enhanced computed tomography scan, 36% (54/150) patients showed pancreatic necrosis. Hct levels were elevated in 58% (55/96) and 61% (33/54) patients with interstitial and necrotizing pancreatitis, respectively. Neither Hct levels at admission nor hemoconcentration at 24 h were associated with the severity, necrosis or OF. Sensitivity, specificity and positive predictive values for both determinations were very low; and negative predictive values were between 61% and 86%, being the highest value for OF.
CONCLUSION: Hct is not a useful marker to predict a worse outcome in acute pancreatitis. In spite of the high negative predictive value of hemoconcentration, the prognosis gain is limited due to an already high incidence of mild disease.
Citation: Remes-Troche JM, Duarte-Rojo A, Morales G, Robles-Díaz G. Hemoconcentration is a poor predictor of severity in acute pancreatitis. World J Gastroenterol 2005; 11(44): 7018-7023
Acute pancreatitis (AP) is an inflammatory process of the pancreas with variable involvement of peripancreatic tissues or remote organ systems. Mostly, it develops as a mild and auto-limited disease, but around 25% of patients present the severe form with an elevated mortality rate (30%), when compared with overall mortality (2-16%)[1,2]. This worrisome condition is due to the eventual development of organ failure (OF) and sepsis[3,4], both complications are associated with the concurrent development of necrotizing pancreatitis that occurs in 20-30% of the cases[2,5]. Severity is currently defined according to the Atlanta International Symposium on AP by the presence of local complications (pancreatic necrosis, pancreatic pseudocyst, and pancreatic abscess) and/or OF (cardiovascular, pulmonary or renal insufficiency, and gastrointestinal bleeding). Several efforts have been made to describe prognostic factors that could help for the identification of high risk patients in order to maintain a closer vigilance, thereby providing a more aggressive medical treatment and an earlier admittance to the intensive care unit.
The usefulness of multiple clinical and laboratory tests to predict severe and/or necrotizing pancreatitis has been studied. Ranson’s criteria are most widely accepted for the assessment of high risk patients; however numerous parameters need to be measured during the first 48 h after admission[9,10]. Other scales like Glasgow or APACHE II are commonly used, but these also require several measurements and have not been proven superior to Ranson’s criteria[11,12]. More recently, biochemical markers, such as C-reactive protein, polymorphonuclear elastase, interleukin-6 and trypsinogen activation peptide[15,16], have been used as predictors of severity in AP. C-reactive protein is a useful marker only 48 h after the onset of acute episodes[13,14] and overall usefulness of the remaining markers is restricted by their limited availability or elevated cost. Thus, so far, no early, accessible and economical predictive marker for severe AP has yet been described.
Hematocrit (Hct) is routinely assessed in every AP case at admission and is an accessible and low-cost test. Recent studies have proposed that hemoconcentration may constitute a good marker for severity of AP, but others were unable to find a significant correlation with the development of OF, pancreatic necrosis or death[17-20]. Thus, the value of hemoconcentration in the initial assessment of AP patients and its implications in prognosis remain controversial. We, therefore, aimed to determine whether hemoconcentration at admission and in the following 24 h was associated with the development of severe AP, pancreatic necrosis and/or OF.
MATERIALS AND METHODS
Patients with a first AP episode admitted consecutively to a tertiary medical center between June 1998 and December 2001 were included in this study. AP diagnosis was confirmed by typical clinical presentation and an increase in amylase or lipase concentration at least thrice the upper limit of normal, and/or evidence of pancreatic inflammation revealed by contrast-enhanced abdominal computed tomography. Medical records of all the patients were reviewed retrospectively for the following variables: gender, age, etiology, Hct level at admission, Hct level at 24 h after admission, development of OF, and severity of AP (both defined according to Atlanta’s criteria), evidence of necrosis in contrast-enhanced abdominal computed tomography, total hospital stay and mortality. Exclusion criteria included patients with previous AP episode(s) or with a first AP episode previously treated in other institutions.
Hematocrit levels at admission and 24 h later were compared with the severity of the pancreatitis, the presence of necrosis or OF. Categorical variables are expressed as absolute and relative frequencies and continuous variables as mean±SD. Receiver operator characteristic (ROC) curves were plotted for the range of Hct levels. Hemoconcentration was defined as an Hct level >44% for male and >40% for female patients[17-20]. The t test was used to analyze continuous variables, whereas the χ2 or F tests were used on categorical variables, when appropriate. A P value <0.05 was considered statistically significant. Statistical analysis was performed using commercially statistical software SPSS 10 (SPSS, Chicago, IL, USA) and NCSS-2000 (NCSS, Kaysville, UT, USA).
Three hundred and thirty-six AP cases were included in the current study. Mean age was 45±17 (range, 15-90 years) years and 55% (n = 185) of patients were females. Sixteen patients (4.7%) were anemic according to reference values established for our population. Mean Hct levels at admission were 41±6 for women and 46±7 for men (P = 0.00001). Biliary disease was the most common cause of the acute episode (n = 148, 44%), followed by alcohol abuse (n = 48, 14%). Other causes included: hypertriglyceridemia (9%), post endoscopic retrograde cholangiopancreatography (7%), drugs-induced (5%), post surgery (4%), obstructive disease (3%), hypercalcemia (1%), trauma (1%) and vasculitis (1%). Thirty-eight events were considered idiopathic (11%). When divided according to gender, biliary disease was more frequent in women and alcoholic pancreatitis was more frequent in men [odds ratio (OR) = 2.16; 95%CI 1.3-3.5 and OR = 40; 95%CI 9.7-243, respectively].
A mild AP episode was diagnosed in 258 (77%) patients, while the remaining 78 (23%) suffered from a severe attack. Organ failure developed in 45 (13%) patients. A contrast-enhanced abdominal computed tomography was performed during the first week after admission to assess the presence of necrosis in 150 cases. The Hct was determined in 233 (69%) patients 24 h after admission; of these, 183 patients (79%) presented with mild AP and 50 (21%) with severe AP. No differences were found in Hct levels at admission regardless of the presence of severe AP, necrosis or OF. A significant decrease in Hct levels was noted in all the patients at 24 h after admission, which was found to be independent of the severity status (Figure 1). There were no differences between the necrotizing and interstitial pancreatitis groups in terms of the fall in Hct levels after admission (7.5±4% vs 6.5±4%, respectively).
Figure 1 Mean hematocrit values at admission and 24 h later.
A ROC curve analysis for several cut offs of Hct levels at admission failed to show a single point combining good sensitivity, specificity, positive and negative predictive values for the detection of severe AP, necrotizing pancreatitis or OF (Figure 2).
Figure 2 Receiver operating characteristic (ROC) curves for several hematocrit cut-off levels on admission as prognostic factors for severity (A), pancreatic necrosis (B) and organ failure (C).
However, the optimal cut-off values of Hct were similar to those used to define hemoconcentration (>44% for males and >40% for females). Neither the presence nor the absence of hemoconcentration at admission was associated to severity, necrosis or OF (Table 1). Hemoconcentration was present in 58% (55/96) and 61% (33/54) of patients with interstitial and necrotizing pancreatitis, respectively. Hemoconcentration at 24 h was not associated to severity, necrosis or OF (Table 2). Hemoconcentration at admission and 24 h later had very low sensitivity, specificity, and positive predictive values for severity, necrosis or OF. Better results were obtained for negative predictive values (Table 3).
Table 1 Hemoconcentration at admission as a marker for severe acute pancreatitis (AP), necrotizing AP and organ failure.
Hospital and intensive care unit stays were higher in patients with severe AP than mild cases (18±15 and 8±3 d vs 10±7 and 0.5±0.1 d, P<0.05). Mortality was significantly higher in patients with severe AP (13% vs 0.03%, P = 0.001).
One of the most important actions to cope with patients suffering from AP is to quickly and accurately assess the severity of the attack. Earlier identification of patients at risk of developing pancreatic necrosis and OF potentially improves their care via prompt admission to intensive care unit[23-25].
Besides the development of several prognostic systems for severity in AP, there are multiple biochemical tests[13-16] and clinical parameters[8,9,26-29] proposed as single markers for severe or necrohemorrhagic pancreatitis. Most of the laboratory assays have significant limitations in clinical practice mainly because they are expensive and not widely available. The clinical features might be the most economical and easily available parameters because they result from routine patient assessment. Among them, the presence of older age, alcohol etiology, time interval between onset of symptoms and admission, rebound tenderness/guarding, obesity, and android fat distribution have been associated with the subsequent development of severe AP. The identification of these parameters as risk factors for the development of severe AP contributed to the understanding of the disease, but their impause of several drawbacks, such as biased clinical data obtainment, controversial results and low sensitivity and/or specificity, and the results have been controversial.
AP is considered as a consequence from an insult to the pancreatic parenchyma that generates a local inflammatory reaction which then propagates and gives place to a generalized inflammatory response. Multiple studies have underlined the role of cytokines (e.g., tumor necrosis factor-α and interleukin-1, -6, and -8) and other inflammatory response mediators (e.g., platelet activating factor) in this propagating process. Interestingly, the inflammatory response is always accompanied by the increase in vascular permeability that produces extravasation of intravascular fluid into the peritoneal cavity[31,32]. The fluid loss significantly decreases the perfusion pressure into the pancreas leading to microcirculatory changes that contribute to pancreatic necrosis. Thus, it has been proposed that hemoconcentration resulting from this fluid loss might well be associated with AP severity.
Gray and Rosenman in 1965 reported that hemoconcentration at admission was a poor prognostic sign in patients with AP, but Talamini et al did not find significant differences of Hct levels obtained within 24 h of admission in survivors and non-survivors of AP. On the other hand, the classic study of Ranson found that a fall in Hct level by greater than 10% during the initial 48 h of therapy correlates with severity and mortality. Thereafter, Baillargeon et al in a retrospective study reported that an admission Hct ≥47% or, opposed to Ranson’s finding, a failure of Hct to decrease at 24 h were predictive of necrosis but not of OF. The same group of authors in a subsequent prospective study of 128 patients with AP established an admission Hct ≥44% and a failure to decrease after 24 h as the best binary predictor for necrosis and OF. Both studies may have a referral bias in patient selection because the included patients transferred from other hospitals may correspond to sicker cases with a delay or less vigorous hydration. In some of the subsequent studies[36,37], elevated values of admission Hct were reported in necrotizing pancreatitis and also associated with serious complications but in all the studies, neither hemoconcentration at admission nor an Hct not decreasing after 24 h were able to predict severity (Table 4). In the current study, we could not find differences in the admission Hct between severe and mild cases. In every case, independently of the presence or absence of necrosis and/or systemic complications, there was a significant decrease in Hct levels at 24 h after admission. This finding might be explained by the common practice of aggressive fluid resuscitation in most of our patients with AP. The mean Hct fall at 24 h was under 10%, being non-significantly lower in necrotizing pancreatitis as expected in agreement with Ranson. According to our results, the accuracy of hemoconcentration at admission and at 24 h later as a marker for severe AP, necrotizing pancreatitis or OF lies in its high negative predictive value, mainly for OF (85% and 86%, respectively), as has been found in the previous reports (Table 4).
Table 4 Prognostic values of hemoconcentration in previous studies.
We analyzed a series of consecutive patients, including 4.7% of them with anemia, in order to test the utility of the Hct in a realistic clinical setting as was done by Khan et al. Our results were similar to those found by them, unlike our higher mean Hct levels. Admission Hct was analyzed separately in males and females as done by Lankisch et al, but in addition to their approach, we also analyzed the Hct at 24 h after admission. Since Hct levels may differ according to atmospheric oxygen pressure (higher in high altitudes) and to gender (lower in females), we constructed ROC curves that displayed cut-off values of Hct to define hemoconcentration at risk for severity in agreement with those previously reported by others[17-20]. Thus, anemia does not seem to play a role in the poor predictive accuracy of Hct in our study.
Our study is the only one that proves that hemoconcentration analyzed according to gender at admission and at 24 h is not a good predictor for severity in AP. We consider that our findings of the poor prognostic value of Hct at admission in AP cannot be attributed to sample bias. However, this is a retrospective study and only 69% of the patients had Hct determination at 24 h after admission; thus the lack of utility of the Hct at this time cannot be established strongly.
In conclusion, the sole clinical application that follows from the current study and several previous reports could be that patients without hemoconcentration have a very low likelihood of developing pancreatic necrosis or organ failure. However, we consider that in spite of the consistent findings of high negative predictive value of hemoconcentration for necrosis and/or OF, there is no prognostic gain because of the pre-existing 75% prevalence of mild AP.
Co-first-authors: José M. Remes-Troche and Guillermo Robles-Díaz
Co-correspondent: José M. Remes-Troche
Science Editor Kumar M and Guo SY Language Editor Elsevier HK
Halonen KI, Leppaniemi AK, Puolakkainen PA, Lundin JE, Kemppainen EA, Hietaranta AJ, Haapiainen RK. Severe acute pancreatitis: prognostic factors in 270 consecutive patients.Pancreas. 2000;21:266-271.
Tenner S, Sica G, Hughes M, Noordhoek E, Feng S, Zinner M, Banks PA. Relationship of necrosis to organ failure in severe acute pancreatitis.Gastroenterology. 1997;113:899-903.
Sakorafas GH, Tsiotou AG. Etiology and pathogenesis of acute pancreatitis: current concepts.J Clin Gastroenterol. 2000;30:343-356.
Baron TH, Morgan DE. Acute necrotizing pancreatitis.N Engl J Med. 1999;340:1412-1417.
Bradley EL. A clinically based classification system for acute pancreatitis. Summary of the International Symposium on Acute Pancreatitis, Atlanta, Ga, September 11 through 13, 1992.Arch Surg. 1993;128:586-590.
McKay CJ, Imrie CW. Staging of acute pancreatitis. Is it important?Surg Clin North Am. 1999;79:733-743.
Lankisch PG, Blum T, Maisonneuve P, Lowenfels AB. Severe acute pancreatitis: when to be concerned?Pancreatology. 2003;3:102-110.
Ranson JH, Rifkind KM, Roses DF, Fink SD, Eng K, Spencer FC. Prognostic signs and the role of operative management in acute pancreatitis.Surg Gynecol Obstet. 1974;139:69-81.
Ranson JH. Etiological and prognostic factors in human acute pancreatitis: a review.Am J Gastroenterol. 1982;77:633-638.
Blamey SL, Imrie CW, O'Neill J, Gilmour WH, Carter DC. Prognostic factors in acute pancreatitis.Gut. 1984;25:1340-1346.
Wilson C, Heath DI, Imrie CW. Prediction of outcome in acute pancreatitis: a comparative study of APACHE II, clinical assessment and multiple factor scoring systems.Br J Surg. 1990;77:1260-1264 DOI : 10.1002/bjs.1800771120.
Chen CC, Wang SS, Lee FY, Chang FY, Lee SD. Proinflammatory cytokines in early assessment of the prognosis of acute pancreatitis.Am J Gastroenterol. 1999;94:213-218.
Ikei S, Ogawa M, Yamaguchi Y. Blood concentrations of polymorphonuclear leucocyte elastase and interleukin-6 are indicators for the occurrence of multiple organ failures at the early stage of acute pancreatitis.J Gastroenterol Hepatol. 1998;13:1274-1283.
Neoptolemos JP, Kemppainen EA, Mayer JM, Fitzpatrick JM, Raraty MG, Slavin J, Beger HG, Hietaranta AJ, Puolakkainen PA. Early prediction of severity in acute pancreatitis by urinary trypsinogen activation peptide: a multicentre study.Lancet. 2000;355:1955-1960.
Khan Z, Vlodov J, Horovitz J, Jose RM, Iswara K, Smotkin J, Brown A, Tenner S. Urinary trypsinogen activation peptide is more accurate than hematocrit in determining severity in patients with acute pancreatitis: a prospective study.Am J Gastroenterol. 2002;97:1973-1977.
Baillargeon JD, Orav J, Ramagopal V, Tenner SM, Banks PA. Hemoconcentration as an early risk factor for necrotizing pancreatitis.Am J Gastroenterol. 1998;93:2130-2134.
Brown A, Orav J, Banks PA. Hemoconcentration is an early marker for organ failure and necrotizing pancreatitis.Pancreas. 2000;20:367-372.
Whitcomb DC, Pedroso M, Oliva J, Venkatesan T, Ulrich C, Saul M. An admission hematocrit of 40 or less predicts a low risk of pancreatic necrosis and may reduce the need for diagnostic CT scan.Gastroenterology. 1999;116:A1176, G 50967.
Lankisch PG, Mahlke R, Blum T, Bruns A, Bruns D, Maisonneuve P, Lowenfels AB. Hemoconcentration: an early marker of severe and/or necrotizing pancreatitis? A critical appraisal.Am J Gastroenterol. 2001;96:2081-2085.
Balthazar EJ, Ranson JH, Naidich DP, Megibow AJ, Caccavale R, Cooper MM. Acute pancreatitis: prognostic value of CT.Radiology. 1985;156:767-772.
Ruíz-Argüelles GJ, Sánchez-Medal L, Loría A, Piedras J, Córdova MS. Red cell indices in normal adults residing at altitude from sea level to 2670 meters.Am J Hematol. 1980;8:265-271.
Klar E, Foitzik T, Buhr H, Messmer K, Herfarth C. Isovolemic hemodilution with dextran 60 as treatment of pancreatic ischemia in acute pancreatitis. Clinical practicability of an experimental concept.Ann Surg. 1993;217:369-374.
Bassi C, Falconi M, Talamini G, Uomo G, Papaccio G, Dervenis C, Salvia R, Minelli EB, Pederzoli P. Controlled clinical trial of pefloxacin versus imipenem in severe acute pancreatitis.Gastroenterology. 1998;115:1513-1517.
Pederzoli P, Bassi C, Vesentini S, Campedelli A. A randomized multicenter clinical trial of antibiotic prophylaxis of septic complications in acute necrotizing pancreatitis with imipenem.Surg Gynecol Obstet. 1993;176:480-483.
McMahon MJ, Playforth MJ, Pickford IR. A compaative study of methods for the prediction of severity of attacks of acute pancreatitis.Br J Surg. 1980;67:22-25.
Werner H-M, Blum T, Haack U, Mahlke R, Lübbers H, Struckmann K, Brinkmann G, Maisonneuve P, Lowenfels AB, Lankisch PG. Acute pancreatitis: Limited value of physical examination for the prediction of pancreatic necrosis.Pancreatology. 2002;2:295-296.
Suazo-Baráhona J, Carmona-Sánchez R, Robles-Díaz G, Milke-García P, Vargas-Vorácková F, Uscanga-Domínguez L, Peláez-Luna M. Obesity: a risk factor for severe acute biliary and alcoholic pancreatitis.Am J Gastroenterol. 1998;93:1324-1328.
Mery CM, Rubio V, Duarte-Rojo A, Suazo-Barahona J, Peláez-Luna M, Milke P, Robles-Díaz G. Android fat distribution as predictor of severity in acute pancreatitis.Pancreatology. 2002;2:543-549.
Gomez-Cambronero LG, Sabater L, Pereda J, Cassinello N, Camps B, Vina J, Sastre J. Role of cytokines and oxidative stress in the pathophysiology of acute pancreatitis: therapeutical implications.Curr Drug Targets Inflamm Allergy. 2002;1:393-403.
Eibl G, Hotz HG, Faulhaber J, Kirchengast M, Buhr HJ, Foitzik T. Effect of endothelin and endothelin receptor blockade on capillary permeability in experimental pancreatitis.Gut. 2000;46:390-394 DOI : 10.1136/gut.46.3.390 PMCid: PMC1727839.
Foitzik T, Eibl G, Hotz HG, Faulhaber J, Kirchengast M, Buhr HJ. Endothelin receptor blockade in severe acute pancreatitis leads to systemic enhancement of microcirculation, stabilization of capillary permeability, and improved survival rates.Surgery. 2000;128:399-407.
Bassi D, Kollias N, Fernandez-del Castillo C, Foitzik T, Warshaw AL, Rattner DW. Impairment of pancreatic microcirculation correlates with the severity of acute experimental pancreatitis.J Am Coll Surg. 1994;179:257-263.
Gray SH, Rosenman LD. Acute pancreatitis. The significance of hemoconcentration at admission to the hospital.Arch Surg. 1965;91:485-489.
Talamini G, Bassi C, Falconi M, Sartori N, Frulloni L, Di Francesco V, Vesentini S, Pederzoli P, Cavallini G. Risk of death from acute pancreatitis. Role of early, simple "routine" data.Int J Pancreatol. 1996;19:15-24.
Pezzilli R, Morselli-Labate AM. Hematocrit determination (HCT) as an early marker associated with necrotizing pancreatitis and organ failure.Pancreas. 2001;22:433-435.
Goulis IG, Koumpoudis P, Arvanitakis C. Hemoconcentration is an early marker of severe and/or necrotizing pancreatitis.Gastroenterology. 2002;122:A366.