DNA end binding activity and Ku70/80 heterodimer expression in human colorectal tumor

Figure 4 Representative immunoblot experiments on nuclear extracts from adenoma cases p70 and p86 protein expression was evaluated by Western blot, on normal (n) and pathologic (t) nuclear extracts from adenoma patients (A1-A7). The p86 protein was slightly detectable on this and on the other filters analyzed. CaCo-2 cell line nuclear extracts (CaCo) were used as internal standard. Filters were reprobed with anti-β-actin monoclonal antibody to normalize the protein levels.