Toll-like receptor 4 plays an anti-HBV role in a murine model of acute hepatitis B virus expression

Figure 4 iNOS expression in the liver of C3H/HeN or C3H/HeJ mice after hydrodynamic-injection of pHBV3. 6. Groups of four C3H/HeN (C, D) or C3H/HeJ (G, H) mice were injected intravenously with 10 μg of pHBV3.6 by hydrodynamics-based transfection. Serial sections of frozen liver tissue were made and stained with anti-HBs antibody (B, D, F, H) or anti-iNOS antibody (A, C, E, G). Red color was developed and indicated the positive staining. The insets in (C), (D), (G), and (H) represent twofold magnification of positive staining. A, B, E, F, saline injected control mice; C, D, G, H, day 2 post injection (original magnification ×200).