Toll-like receptor 4 plays an anti-HBV role in a murine model of acute hepatitis B virus expression

Figure 1 TLR4 expression in the liver of C3H/HeN mice after hydro-dynamic injection of pHBV3. 6. Groups of four C3H/HeN mice were injected intravenously with 10 μg of plasmid by hydrodynamics-based transfection. The liver tissues were collected at day 3 post injection and 4-μm cryosections were made, stained with anti-HBsAg-FITC and anti-TLR4-PE antibody. The green represents EGFP-positive (E) or HBsAg-positive cells (I) and the red represents TLR4-positive cells (B, F, J). The blue represents counter staining by Hoechst 33258 dye (C, G, K). A-D: naïve; E-H: pEGFP-N1; I–L: pHBV3.6. The arrows indicate the HBsAg positive hepatocytes and the arrowheads indicate the TLR4 positive immune cells (original magnification ×200)