Detection of germline mutations of hMLH1 and hMSH2 based on cDNA sequencing in China

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Nov 14, 2005 (publication date) through Apr 26, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Colorectal Cancer

World J Gastroenterol. Nov 14, 2005; 11(42): 6620-6623
Published online Nov 14, 2005. doi: 10.3748/wjg.v11.i42.6620

Table 2 hMLH1 and hMSH2 primers used for sequencing of cDNA

Sense	Antisense
hMLH1-1F CCTGGCTCTTCTGGCGCC	hMLH1-1R CTTTTCTCCTCGTGGCTATGTTGT
hMLH1-2F ATGTGCTGGCAATCAAGGGA	hMLH1-2R GGTGCACATTAACATCCACATTCT
hMLH1-3F CCAAAAACACACACCCATTCCT	hMLH1-3R CCTTTGTTGTATCCCCCTCCA
hMLH1-4F GCTGAAGTGGCTGCCAAAAAT	hMLH1-4R CATCTTCCTCTGTCCAGCCACTC
hMLH1-5F TTGCCATGCTTGCCTTAGATAGTC	hMLH1-5R GAGCGCAAGGCTTTATAGACAATG
hMLH1-6F GCTCCATTCCAAACTCCT	hMLH1-6R TATGTTAAGACACATCTATTTATTTA
hMSH2-1F GGCGGGAAACAGCTTAGTGGG	hMSH2-1R CTCTGGCCATCAACTGCGGAC
hMSH2-2F GGCTTCTCCTGGCAATCTCTCTCA	hMSH2-2R CTTGATTACCGCAGACAGTGATGAAAC
hMSH2-3F GCAAAAAGGGAGAGCAGATGAATAGTG	hMSH2-3R GGCAAGTCGGTTAAGATCTGGGAAT
hMSH2-4F AGATGCAGAATTGAGGCAGACTTTACA	hMSH2-4R GGACTTTTTCTTCCTTACAGGTTACACG
hMSH2-5F CAGAGATCTTGGCTTGGACCCT	hMSH2-5R TTCAACACAAGCATGCCTGGAT
hMSH2-6F CGTGTCAAATGGAGCACCTGTTC	hMSH2-6R GATTGGCCAAGGCAGTAAGTTCAT
hMSH2-7F AATCATAGATGAATTGGGAAGAGGAACT	hMSH2-7R GGGCATTTGTTTCACCTTGGAC
hMSH2-8F CTATCTGGAAAGAGAGCAAGGTGAA	hMSH2-8R CCACAAACTACATGATTTTATTTATAAAATTC

Citation: Wang CF, Zhou XY, Zhang TM, Sun MH, Shi DR. Detection of germline mutations of hMLH1 and hMSH2 based on cDNA sequencing in China. World J Gastroenterol 2005; 11(42): 6620-6623
URL: https://www.wjgnet.com/1007-9327/full/v11/i42/6620.htm
DOI: https://dx.doi.org/10.3748/wjg.v11.i42.6620