Detection of germline mutations of hMLH1 and hMSH2 based on cDNA sequencing in China

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Nov 14, 2005 (publication date) through Apr 25, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Colorectal Cancer

World J Gastroenterol. Nov 14, 2005; 11(42): 6620-6623
Published online Nov 14, 2005. doi: 10.3748/wjg.v11.i42.6620

Table 1 Sequence and localization of primers used for amplification of cDNA of hMLH1 and hMSH2

Sense	Antisense
hMLH1-1F(1-18)	hMLH1-5R(2198-2175)
CTTGGCTCTTCTGGCGCC	GAGCGCAAGGCTTTATAGACAATG
hMLH1-4F(1333-1353)	hMLH1-6R(2484-2459)
GCTGAAGTGGCTGCCAAAAAT	TATGTTAAGACACATCTATTTATTTA
hMSH2-1F(1-21)	hMSH2-7R(2753-2732)
GGCGGGAAACAGCTTAGTGGG	GGGCATTTGTTTCACCTTGGAC
hMSH2-6F(1898-1920)	hMSH2-8R(3145-3114)
CGTGTCAAATGGAGCACCTGTTC	CCACAAACTACATGATTTTATTTATAAAATTC

Citation: Wang CF, Zhou XY, Zhang TM, Sun MH, Shi DR. Detection of germline mutations of hMLH1 and hMSH2 based on cDNA sequencing in China. World J Gastroenterol 2005; 11(42): 6620-6623
URL: https://www.wjgnet.com/1007-9327/full/v11/i42/6620.htm
DOI: https://dx.doi.org/10.3748/wjg.v11.i42.6620