Modulation of gene expression in MHCC97 cells by interferon alpha

Table 1 Primer sequence and condition for PCR analysis of selected genes

Category	Gene	Sense and antisense primers	Annealing(°C)	Cycles	Size(bp)
Cytoskeletal gene	Neutral calponin	5’-TGGCACCAGCTAGAAAACCT-3’; 5’-CAGGGACATGGAGGAGTTGT-3’	56	26	498
Proliferative gene	hMCM2	5’-ACCGAGACAATGACCTACGG-3’; 5’-CTAGCTGTCTGCCCCTTGTC-3’	56	30	382
Angiogenic gene	VEGF165 receptor	5’-GAAGCACCGAGAGAACAAGG-3; 5’-CACCTGTGAGCTGGAAGTCA-3’	56	30	359
IFN-α-induced genes	9-27	5’-TTGGTCCCTGGCTAATTCAC-3’; 5’-ATGAGGATGCCCAGAATCAG-3’	53	35	491
	ISG-56 ku	5’-AAAAGCCCACATTTGAGGTG-3’; 5’-GGCTGATATCTGGGTGCCTA-3’	54	30	451
MAPK pathway-related genes	ERK activator kinase (MEK2)	5’-CGAAAGGATCTCAGAGCTGG-3’; 5’-GTGCTTCTCTCGGAGGTACG-3’	56	26	349
	G3BP2	5’-GCAGAACCTGTTTCTCTGCC-3’; 5’-CACCACCACCTCTGGTTTCT-3’	56	30	475
	CHED	5’-TCCTTGGCGAACTCTTCACT-3’; 5’-TGCCATAAAGGGAGATCTGG-3’	56	30	336
cAMP/PI3 pathway-related gene	Adenylyl cyclase	5’-CCAGGAGCCTGAAGAATGAG-3’; 5’-GGCTTCTGAGCTCCAATCAC-3’	53	35	439
Housekeeping gene	γ-Actin	5’-ATGGAAGAAGAAATCGCCGC-3’; 5’-ACACGCAGCTCGTTGTAGAA-3’	55	25	287