Endothelial adhesion of synchronized gastric tumor cells changes during cell cycle transit and correlates with the expression level of CD44 splice variants

Figure 4 Expression of CD44v4(A), CD44v5(B) and CD44v7(C) on MKN45 cells during cell cycle transit. Synchronized MKN45 cells were labeled with anti-CD44v4, anti-CD44v5 or anti-CD44v7 at indicated time points. Non-synchronized cells served as controls. Fluorescence intensity was measured using a FACscan (FL-1H (log) channel histogram analysis; 1 x104 cells/scan) and expressed as mean fluorescence units (MFU). Mean±SD of n = 6 experiments.