Endothelial adhesion of synchronized gastric tumor cells changes during cell cycle transit and correlates with the expression level of CD44 splice variants

Figure 2 Adhesion capacity of synchronized MKN45 cells to HUVEC. MKN45 cells were treated for 24 h with aphidicolin. Cells were then washed and replated in fresh medium to allow cell cycle progression. Cells were sampled after 0, 4, 8 and 16 h following aphidicolin treatment (n = 6). A 60-min adhesion to HUVEC was determined at each time point and compared with non-synchronous cells. Mean±SD of n = 6 experiments.