Endothelial adhesion of synchronized gastric tumor cells changes during cell cycle transit and correlates with the expression level of CD44 splice variants

Figure 1 Facs analysis of synchronized MKN45 cells. Cell number is given in %. Non-synchronized control cells reside in the S-phase of the cell cycle. When MKN45 cells were blocked with aphidicolin and then replated in fresh medium to allow cell cycle progression, MKN45 entered S phase after 4 h (arrow a), G2/M after 8 h (arrow b) and G0/G1 after 16 h (arrow c). Mean±SD of n = 3 experiments (A,B).