Role of nuclear receptor CAR in carbon tetrachloride-induced hepatotoxicity

Figure 1 (A) PB pretreatment induced CCl₄ toxicity. CAR^+/+ or CAR^–/– mice were administered a 25-mg/kg dose of CCl₄ by intraperitoneal injection with or without PB pretreatment (n=6 per treatment group). Serum was collected and ALT levels were measured after 24 h. The ALT level was slightly higher in CAR^+/+ mice than in CAR^–/– mice with CCl₄ treatment (P<0.001). The difference in ALT levels became clearer between CAR^+/+ and CAR^–/– mice with pre-treatment by PB (P<0.001). (B) Dose dependency of CCl₄ toxicity. CAR^+/+ and CAR^–/– mice were given 25-, 50-, or 100-mg/kg doses of CCl₄. Blood samples were collected 24 h later, and serum ALT levels were measured (n=4). CAR^–/– animals were significantly less sensitive than CAR^+/+ mice to CCl₄ toxicity (P<0.001). Data are mean±SD.