Cancer-derived VEGF plays no role in malignant ascites formation in the mouse

Figure 2 Effect of VEGF knockdown on cell proliferation. A: 3×10⁴ VEGF-KD7 or VEGF-WT cells were grown in 24-well plates, and the numbers of viable cells were assessed using the MTT assay at 24 (P = 0.082) and 48 h (P = 0.078) (n = 4, mean±SD); B: 2×10⁶ VEGF-KD7 or VEGF-WT cells were injected into C57BL/6 mice subicutaneous, the tumor size was measured, and the volume was calculated as [(length (mm)×width (mm)²)]/2 (n = 4, P = 0.18, mean±SD).