Screening and identification of proteins interacting with nucleostemin

Figure 3 Co-immunoprecipitation of protein NS and PPP2R5A. Total proteins from cells co-transferred with plasmids pCDNA3-NS and pcDNA3-myc-PPP2R5A were used for immunoprecipitation with antibody against NS or c-myc and antibody against c-myc or NS was used in Western blot. Mouse IgG was used in immunoprecipitation as negative control.